Flow Cytometry

The APL includes state-of-the-art facilities for the measurement and enumeration of cellular populations. The lab has access to both image-based (FlowSight) and traditional (easyCyte 12HT) flow cytometry. The FlowSight is capable of 10-color detection along with bright field images at a resolution of 0.5 um. The easyCyte 12HT is capable of 14-color high throughput (HT) processing. The flow cytometers are capable of detecting flurochromes excited off the violet (405 nm), blue (488 nm), and/or red (642 nm) lasers. All instruments feature 96-well plate handling for high-throughput analysis and walk away processing. Additional supporting equipment is available to facilate 96-well high-throughput sample processing. Seperate, dedicated workstation computers are available for processing of raw data files using either FCS Express, Incyte, or IDEAS software.